e-learning
Pangenome analysis with Roary
Abstract
Pangenomics
About This Material
This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.
Questions this will address
- How can we identify core and accessory genes across multiple bacterial strains?
- How do we generate a pangenome using Prokka and Roary?
- How can we infer the evolutionary relationship of these strains?
Learning Objectives
- Annotate genomes using Prokka.
- Use Roary to identify core and accessory genes.
- Construct a phylogenetic tree of core genes with IQ-TREE.
Licence: Creative Commons Attribution 4.0 International
Keywords: Genome Annotation, pangenome, phylogeny, prokaryote
Competency level: • Beginner
Target audience: Students
Resource type: e-learning
Version: 1
Status: Active
Prerequisites:
Introduction to Galaxy Analyses
Learning objectives:
- Annotate genomes using Prokka.
- Use Roary to identify core and accessory genes.
- Construct a phylogenetic tree of core genes with IQ-TREE.
Date modified: 2026-07-21
Date published: 2026-07-21
Authors: 
Niklas Mayle, Saim Momin
Scientific topics: Genomics
External resources:
Activity log
